Journal: Journal of visualized experiments : JoVE

Article Title: Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia

doi: 10.3791/66093

Figure Lengend Snippet: Table of Materials

Article Snippet: Name of Material/ Equipment Company Catalog Number Comments/Description 1 mL pipette Tips Genesee Scientific 24-165RL 1.7 mL Microcentrifuge Tubes AVANT L211511-CS 10 μL pipette Tips Genesee Scientific 24-140RL 10 mL Individually Wrapped Sterile Serological Pipettes Globe scientific 1760 1000 mL Vacuum Filtration Flask NEST 344021 15 mL Centrifuge Tube VWR 10026-076 2 mL Aspirating Pipette NEST 325011 200 μL pipette Tips Genesee Scientific 24-150-RL 25 mL Individually Wrapped Sterile Serological Pipettes Globe scientific 1780 5 mL Individually Wrapped Sterile Serological Pipettes Globe scientific 1740 5 mL Polystyrene Round-Bottom Tube 12 x 75 mm style Falcon 352054 5 mL Polystyrene Round-Bottom Tube with Cell Strainer Cap 12 x 75 mm style Falcon 352235 50 mL Centrifuge Tube NEST 602052 6 Well, Flat Bottom with Low Evaporation Lid Falcon 353046 Absorbent Underpads with Waterproof Moisture Barrier VWR 56616-031 APC MicroBeads Miltenyi 130-090-855 2 mL autoMACS Pro Separator Miltenyi Biotec GmBH 4425745 BD Pharmingen TM Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block TM ) BD Biosciences 553141 0.5 mg/mL Bovine Serum Albumin Sigma-Aldrich A7906 66.000 g/mol Brilliant Violet 421 TM anti-mouse Ly-6A/E (Sca-1) Antibody (D7) Invitrogen 404-5981 0.2 mg/mL C57BL/6J Mice Jackson Laboratory 664 Carbon Dioxide Gas Tank Airgas CD50 CD31 (PECAM-1) Monoclonal Antibody (390), PE-Cyanine7 Invitrogen 25-0311-82 0.2 mg/mL CD45 Monoclonal Antibody (30-F11), APC Invitrogen 17-0451-82 0.2 mg/mL Cell Strainer 70 μm Nylon Falcon 352350 Cole-Parmer Essentials Mortar and Pestle; Agate, 125 mL Cole-Parmer EW-63100-62 Collagenase from Clostridium histolyticum Sigma-Aldrich C5138-500MG 1 mg/mL Collagenase Type I STEMCELL 7415 0.25% in 1 mL 20% FBS/DPBS Corning Mini Centrifuge CORNING 6770 Corning Stripettor TM Ultra Pipet Controller Corning 4099 Deoxyribonuclease I from bovine pancreas Sigma-Aldrich D4513 1 mg/mL Dispase II, powder Gibco 117105041 2 mg/mL DPBS 10X gibco 14200-075 eBioscience TM 1X RBC Lysis Buffer Invitrogen 00-4333-57 Ethanol absolute, KOPTEC, meets analytical specification of BP, Ph.

Techniques: Transferring, Sterility, Suction Filtration, Evaporation, Purification, Blocking Assay, Red Blood Cell Lysis